Prolactin inhibits corticoid-induced differentiation of active na+
transport across cultured larval bullfrog skin.
Takada, Makoto, Hideko Yai, and Kazue Takayama-Arita.
Department of Physiology, Saitama Medical School, Moroyama, Iruma
-gun, Saitama 350-04 Japan
APStracts 2:0232C, 1995.
Active Na + transport differentiates in larval bullfrog skin cultured
with corticoids. After 2 weeks in culture, the epidermis became
positive against human blood group antigen A, the marker for the
adult-type cells of the epidermis, but was negative to the antibody
against the acetylcholine receptor (AChR), the marker for the larval
-type epidermis. ? Amiloride (10 -5 M) did not inhibit the
differentiation of active Na + transport. On the other hand, in skin
cultured with prolactin (PRL, 2 [mu] g/ml), the epidermis remained
negative against antigen A and positive against AChR, and the
differentiation of active Na + transport was inhibited. Thyroid
hormone did not antagonize the inhibitory action of PRL on this
transport differentiation. Prolactin affected the basal cells of the
larval epidermis and inhibited development of corticoid-induced adult
features in the epidermis.
Received 29 November 1994; accepted in final form 5 June 1995.
APS Manuscript Number C694-4.
Article publication pending Am. J. Physiol. (Cell Physiology).
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on 6 July 1995.