Lbthe references were done using reference manager utilization and
preferred metabolic pathway of ketone bodies for lipid synthesis by
isolated rat hepatoma cells.
Hildebrandt, Leslie A., Terry Spennetta, Charles Elson, and Earl
Shrago.
Departments of Medicine and Nutritional Sciences, University of
Wisconsin, Madison, WI 53706
APStracts 2:0115C, 1995.
Morris hepatoma 7777 cells freshly isolated from highly malignant
tumors grown in the hind limb of buffalo rats actively convert ketone
bodies to cholesterol and fatty acids. Based on results obtained with
(-)-hydroxy citrate, an inhibitor of the ATP citrate lyase enzyme,
the metabolic pathway for acetoacetate conversion to lipids is
exclusively cytoplasmic, whereas, that for 3-hydroxybutyrate involves
both extra and intramitochondrial compartments. Subcellular
distribution studies indicated accumulation and compartmentation of
3-hydroxybutyryl CoA primarily in the cytoplasm of hepatoma cells
incubated with either ketone body. By contrast, the compart mentation
of acetoacetyl CoA is dependent on whether the substrate is aceto
acetate or 3-hydroxybutyrate. With acetoacetate, the acetoacetyl CoA
is entirely cytoplasmic, whereas, with 3-hydroxybutyrate it is
equally divided between the intra and extramitochondrial
compartments. The results are discussed in terms of the known and
proposed metabolic pathways for lipid synthesis from ketone bodies,
particularly that from 3-hydroxybutyrate.
Received 30 August 1994; accepted in final form 20 December 1994.
APS Manuscript Number C511-4.
Article publication pending Am. J. Physiol. (Cell Physiology).
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on 7 March 1995.