Immortalization of epithelial cells .
Hopfer, Ulrich, James W. Jacobberger, Dieter C. Gruenert, Richard L.
Eckert, Parmjit S. Jat, and Jeffrey A. Whitsett.
Department of Physiology/Biophysics, Case Western Reserve
University, School of Medicine, Cleveland, OH 44106-4970; Cancer
Research Center, Case Western Reserve University, School of Medicine,
Cleveland, OH 44106-4494; Departments of Physiology/Biophysics,
Dermatology, and Biochemistry, Case Western Reserve University,
School of Medicine, Cleveland, OH 44106-4970; Cardiovascular Research
Institute, Department of Laboratory Medicine, Gene Therapy Core
Center University of California, San Francisco, CA 94143; Ludwig
Institute for Cancer Research, UC London Medical School Branch, 91
Riding House Street, London W1P 8BT,UK; Children's Hospital Medical
Center, Division of Pulmonary Biology, Cincinnati, OH 45229
APStracts 2:0176C, 1995.
The methodologies for isolating cell lines have become very powerful,
particularly in terms of retaining differentiated features of the
parents cells. Cell lines can be developed from primary or early
passage cells as well as from transgenic animals that carry an
immortalizing gene. Cell lines from epithelia have been selected for
their polar orientation, tight junction formation, and expression of
differentiated markers or functions. These cell lines provide useful
models for studying cell biology of specific tissues, tumorigenicity,
genetic abnormalities, or to help screen for effective methods of
gene therapy.
Received 10 February 1995; accepted in final form 14 April 1995.
APS Manuscript Number C76-5.
Article publication pending Am. J. Physiol. (Cell Physiology).
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on 2 May 1995.