Stretch overload induced satellite cell activation in slow tonic
muscle from adult and aged japanese quail.
Carson, James A., and Stephen E. Alway.
Neuromuscular Research Laboratory, Department of Exercise Science,
The Ohio State University, Columbus, Ohio 43210
APStracts 2:0326C, 1995.
Stretch overload induced activation of satellite cells in the anterior
latissimus dorsi muscle (ALD) was examined in full grown adult (12
weeks old) and aged (90 weeks old) Japanese quail. 5'-bromo-2'
-deoxyuridine (BrdU) constant release pellets (0.22mg BrdU/g body
wt/day) were implanted subcutaneously prior to weighting the left
wing of each bird. Nuclei that incorporated BrdU were localized by
immunohistochemistry after one or two weeks of stretch overload.
Total fiber number was quantified by counting all fibers in a
histological cross-section from the mid-belly of the ALD. Aging
reduced increases in ALD mass and fiber number during two weeks of
stretch-overload. Fiber proliferation in the ALD of aged birds also
demonstrated an altered time course. The percentage of BrdU positive
nuclei associated with muscle fibers and the percentage of fibers
associated with BrdU positive nuclei, did not differ between age
groups. The altered time course of new fiber formation in the ALD of
aged birds during two weeks of stretch-overload does not appear to be
related to the capability to activate satellite cells.
Received 24 February 1995; accepted in final form 29 August 1995.
APS Manuscript Number C102-5.
Article publication pending Am. J. Physiol. (Cell Physiology).
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on 23 September 1995.