Periprandial systemic and regional lipase activity in normal
man.
Coppack, S. W., T. J. Yost, R. M. Fisher, R. H. Eckel, J. M. Miles.
Endocrine Research Unit, Mayo Clinic, Rochester, MN, University of
Colorado Health Sciences Center, Denver, CO & University College
London Medical School, London, UK
APStracts 2:0248E, 1995.
An assay for plasma lipoprotein lipase activity was used, without
prior injection of heparin, to study arteriovenous differences of
lipases across skeletal muscle and adipose tissue of normal male
volunteers. Lipoprotein lipase (LPL) and hepatic lipase (HTGL)
activities and triglyceride concentrations were measured in arterial
plasma and in venous effluent plasma from forearm skeletal muscle and
subcutaneous abdominal adipose tissue, in the postabsorptive state
and after a mixed meal. Triglyceride clearance by the tissues was
greater across adipose tissue than muscle. There were no
arteriovenous differences for HTGL activity. In the postabsorptive
state skeletal muscle released LPL activity, but adipose tissue did
not. Postprandially the arterial LPL and HTGL activities did not
change. LPL activity in adipose tissue venous effluent rose while
that in muscle venous effluent decreased. These results show that the
release of LPL from subcutaneous adipose and forearm tissues are
regulated differently, reflecting in vivo differences in LPL
regulation at the tissue level.
Received 15 November 1993; accepted in final form 20 November
1995.
APS Manuscript Number E445-3.
Article publication pending Am. J. Physiol. (Endocrinol. Metab.).
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on 12 December 95