Physiologic concentrations of insulin and t3 stimulate 3t3-l1
adipocyte acyl-coa synthetase gene transcription.
Kansara, Mala S., Aruna K. Mehra, Jean Von Hagen, Ella Kabotyansky,
and Pamela J. Smith.
Medical Service, Department of Veterans Affairs Medical Center, E.
Orange, New Jersey, 07019 and the Department of Medicine, University
of Medicine and Dentistry of New Jersey--New Jersey Medical School,
Newark, N.J.
APStracts 2:0252E, 1995.
Acyl-CoA Synthetase (ACS) is a key gene for cellular utilization of
long chain fatty acids. We characterized its regulation by
physiological concentrations of insulin that acutely regulate
metabolism. Our results demonstrate that subnanomolar insulin rapidly
and maximally stimulates ACS gene transcription in the absence of
protein synthesis; 0.5 nM insulin produced a 2.3 + 0.1 -fold increase
in ACS mRNA levels and induced ACS gene transcription 2.4- + 0.3
-fold. The insulin sensitivity of ACS was compared with lipoprotein
lipase (LPL) and stearoyl-CoA desaturase-1 (SCD-1), which were both
were less sensitive to insulin.
Received 9 June 1995; accepted in final form 30 November 1995.
APS Manuscript Number E266-5.
Article publication pending Am. J. Physiol. (Endocrinol. Metab.).
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on 23 December 95