Metabolic fate of an oral long chain triglycerides load in
human.
Binnert, C., C. Pachiaudi, M. Beylot, M. Croset, R. Cohen*, Jp. Riou,
and M. Laville.
INSERM U 197. Facult[acute]e de m[acute]edecine Alexis CARREL. Rue
Guillaume PARADIN. 69372 LYON Cedex 08, Centre de Recherche en
Nutrition Humaine de Lyon. Hopital E. Herriot. Place d'Arsonval.
69437. Lyon Cedex 03 and * Service de Radioanalyse, Centre de
M[acute]edecine Nucl[acute]eaire, 69003 Lyon, France
APStracts 2:0210E, 1995.
In order to determine the steps involved in the metabolism of ingested
triglycerides (TG), ten healthy women were studied during 6 hours
after ingestion of 30 g of olive oil labelled with [1,1,1,-13C3]
triolein. The appearance of 13C was followed in chylomicron-TG (CM
-TG), NEFA, VLDL-TG and in expired gas. Indirect calorimetry was used
to determine total lipid oxidation. After 90 min, labelling was
higher in CM-TG than in NEFA or VLDL. At 180 min, a plateau of
enrichment was obtained for CM-TG and NEFA demonstrating the entry of
exogenous lipids in the NEFA pool. After 300 min a plateau was
observed for VLDL-TG with level of enrichments (0.38+/-0.04%) similar
to those observed for NEFA (0.36+/-0.03%) suggesting a precursor
-product relationship. Only 19+/-2% of the load was oxidized. From 300
to 360 min, 70 % of total lipid oxidation were from exogenous TG. We
conclude that after ingestion of a lipid load, a cycle of fatty
acids-TG occurs from CM to NEFA and from NEFA to VLDL. Furthermore,
this lipid load has a sparing effect on endogenous lipid stores.
Received 24 July 1995; accepted in final form 28 September 1995.
APS Manuscript Number E346-5.
Article publication pending Am. J. Physiol. (Endocrinol. Metab.).
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on 6 November 95