Role of transforming growth factor [beta] in the restitution of
injured guinea pig gastric mucosa in vitro.
Yanaka, Akinori, Hiroshi Muto, Hisayuki Fukutomi, Susumu Ito, and
William Silen.
Department of Gastroenterology, Institute of Clinical, Medicine,
University of Tsukuba, Ibaraki 305, Japan, Department of
Neurobiology, Harvard Medical School, Boston, MA 02115, Department of
Surgery, Beth Israel Hospital, Harvard, Medical School, Boston, MA
02215.
APStracts 2:0259G, 1995.
The role of Transforming growth factor-[beta] (TGF-[beta]) in
restitution was examined in intact sheets of injured guinea pig
gastric mucosa, in which the epithelial cell-collagen interaction can
be quantitatively evaluated. The luminal surface of intact sheets of
in vitro guinea pig gastric mucosa was injured by exposure to 1.25
mol/L NaCl for 10 min. Restitution was evaluated by measurement of
transmucosal electrical resistance and 3H-mannitol flux before and
after injury. Recovery of electrical resistance and 3H-mannitol flux
was retarded by inhibition of endogenous TGF-[beta] with either
aprotinin or anti-TGF-[beta] antibody, effects restored by human
recombinant TGF-[beta]1. During inhibition of endogenous TGF-[beta],
type IV collagen accelerated the recovery. Inhibition of
reconstruction of the basement membrane by simultaneous addition of
cis-4-OH-l-proline and anti-type IV collagen completely abolished the
enhancement of the recovery by TGF-[beta]1. These results suggest
that endogenous TGF-[beta] is required for restitution to occur in
guinea pig gastric mucosa, and that type IV collagen plays an
important role in TGF-[beta]-abetted restitution.
Received 22 May 1995; accepted in final form 28 November 1995.
APS Manuscript Number G220-5.
Article publication pending Am. J. Physiol. (Gastrointest. Liver
Physiology).
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on 23 December 95