Trehalase gene expression during postnatal development of rabbit intestine and kidney. Galand, Guy, Fabienne L'horset, Yvette Longis, Christine Perret. Laboratoire de Physiologie Animale, Facult[acute]e des Sciences, Universit[acute]e de Reims Champagne-Ardenne, 51062 REIMS Cedex, France, INSERM U120, H[circumflex]opital Robert Debr[acute]e, 24 Bvd Serrurier, 75019 Paris, France.
APStracts 2:0136G, 1995.
Rabbit trehalase is a 75 kDa, GPI anchored glycoprotein of the microvillous membrane of the enterocyte and kidney proximal tubule epithelial cells. The purpose of this work was to try to elucidate the molecular basis of trehalase gene expression in intestine and kidney during normal postnatal development and after hydrocortisone injection in suckling rabbits. Trehalase cDNA isolated, sequenced and characterized by Ruf et al. (1990) was used to quantify trehalase mRNA. To measure the amount of trehalase mRNA encoding for trehalase, poly-A mRNA was isolated and analyzed by northern blot hybridization. This cDNA hybridized to a 1.8 kilobase mRNA in the small intestine and kidney. In developing rabbit intestine, after a slow decrease between 4 and 10 days, there is a sharp and parallel rise of both trehalase specific activity (x 28) and mRNA (x 10) between 10 and 30 days after birth. In contrast, in kidney between 4 and 30 days the general developmental profile of both parameter is very different. There is an overall significant and parallel increase of both trehalase specific activity (x 3.3) and mRNA (x 4.3). In intestine the longitudinal gradient of trehalase activity and mRNA expression is different in adult and 16 days cortisol treated suckling rabbits. 

Received 6 September 1994; accepted in final form 17 June 1995.
APS Manuscript Number G335-4.
Article publication pending Am. J. Physiol. (Gastrointest. Liver
Physiology).
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on 18 July 1995.