Triglyceride and cholesterol transport during absorption of
glycerol trioleate versus glycerol trielaidate in rats.
Kalogeris, Theodore J., Laura Gray, Yu-Yan Yeh, and Patrick Tso.
Departments of Physiology and Biophysics, Louisiana State
University, Medical Center, Shreveport, LA 71130, and Nutrition,
Pennsylvania State, University, University Park, PA 16802
APStracts 2:0146G, 1995.
We used the conscious, chronic lymph-fistula rat to compare intestinal
lymphatic transport of glycerol trioleate (TO) versus glycerol
trielaidate (TE), and to determine the effect of TO versus TE on
absorption and transport of cholesterol. Rats were implanted with
intestinal lymph fistulas and duodenal cannulas, then given
intraduodenal infusions of lipid emulsions containing purified TO or
TE (40 [mu]mol/h) and cholesterol (7.8 [mu]mol/h + 2 [mu]Ci 14C
-cholesterol). Lymph samples were collected at 0, 2, 4, 5, 6, 7, and 8
h after the start of lipid infusion. Lymphatic output and luminal and
gut wall recovery of radioactive lipid at 8 h were quantified.
Triglyceride fatty acid isomer did not affect lymphatic output of
triglyceride; lymph TG fatty acid composition and output reflected
infusate composition. Lymphatic output of cholesterol (mass and
radioactivity) was not different between groups; luminal and gut wall
recovery of 14C-cholesterol was also similar between groups. Similar
lymphatic transport of TG and cholesterol between triolein and
trielaidin-infused rats was maintained for up to 16 h after the
cessation of an infused lipid load. These results indicate that TO
and TE are transported into lymph similarly, and that cholesterol
absorption and transport are similar irrespective of whether TO or TE
is the triglyceride source. The data suggest that trans-fatty acid
-induced hypercholesterolemia is not due to altered intestinal
absorption and transport of cholesterol.
Received 7 October 1994; accepted in final form 13 July 1995.
APS Manuscript Number G406-4.
Article publication pending Am. J. Physiol. (Gastrointest. Liver
Physiology).
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on 30 July 1995.