Immunocytes and abnormal gastrointestinal motor activity during ileitis in dogs. Jouet, Pauline, Sushil K. Sarna, Chandar Singaram, Robert P. Ryan, Cecilia J. Hillard, Gordon L. Telford, John Fink, and James D. Henderson. Departments of, Surgery, Physiology, Pharmacology and Toxicology, and Pathology, Medical College of Wisconsin, and Zablocki VA Medical Center, Milwaukee, WI 53295
APStracts 2:0100G, 1995.
Infiltration of specific immunocytes and stimulation of abnormal gastrointestinal motor activity during ileal inflammation induced by mucosal exposure to ethanol and acetic acid was investigated in 17 dogs. Ileal inflammation significantly increased the frequency of giant migrating contractions (GMCs) and decreased the frequency of migrating motor complexes (MMCs). The frequency of retrograde giant contractions (RGCs) increased only on the day of ethanol and acetic acid treatment. Diarrhea, urgency of defecation and apparent abdominal discomfort were related to the increased frequency of GMCs. Ileal inflammation also prolonged the duration of postprandial MMC disruption. Histologic and immunohistochemical findings indicated transmural inflammation with marked increase in polymorphonuclear cells in the lamina propria and muscularis externa layers. Myeloperoxidase activity increased several fold in both layers. Interleukin-2 receptor containing cells increased in the lamina propria. Other immunocytes, such as B and T-lymphocytes, dendritic cells and HLADR-positive cells, did not exhibit a significant increase in the inflamed ileum as compared with the normal proximal jejunum. We conclude that stimulation of GMCs may be the major motility marker of intestinal inflammation. 

Received 27 May 1994; accepted in final form 12 May 1995.
APS Manuscript Number G202-4.
Article publication pending Am. J. Physiol. (Gastrointest. Liver
Physiology).
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on 30 May 1995.