Glycine-extended gastrin potentiates gastrin-stimulated gastric
acid secretion in the rat.
Higashide, Shunichi, Guillermo Gomez, George H. Greeley, Jr., Courtney
M. Townsend, Jr., and James C. Thompson.
Department of Surgery, The University of Texas Medical Branch,
Galveston, Texas 77555-0725
APStracts 2:0217G, 1995.
The purpose of this study was to examine whether an intermediate form
of amidated gastrin, glycine-extended gastrin (Gly-G), can stimulate
gastric acid secretion in conscious rats prepared with gastric
fistulas. Intravenous (IV) administration of Gly-G (20?nmol x kg-1 x
h-1) alone for 2 h did not stimulate gastric acid secretion; however,
administration of Gly-G (2,?20?nmol x kg-1 x h-1) in combination with
a bolus administration of gastrin (9.5 nmol/kg) potentiated acid
secretion significantly. Gastric acid secretion in response to
gastrin alone and gastrin plus Gly-G (2?nmol x kg-1 x h-1) were 109.1
+/- 21.6 and 170.1 +/- 27.7 [mu]Eq x kg-1 x h-1, respectively
(p?<?0.05). Gastric acid secretion in response to gastrin alone
and gastrin plus Gly-G (20 nmol x kg-1 x h-1) were 84.8 +/- 17.5 and
164.1 +/- 29.3 [mu]Eq x kg-1 x h-1, respectively (p?< 0.05). IV
administration of Gly-G (20 nmol x kg-1 x h-1) failed to increase
histamine (1 mg/kg)-stimulated acid output. These results demonstrate
that Gly-G can selectively potentiate the stimulatory effect of
gastrin on acid secretion in rats, and that the unprocessed form of
gastrin, Gly-G, can exert a biological effect in the stomach.
Received 1 September 1995; accepted in final form 20 October
1995.
APS Manuscript Number G385-5.
Article publication pending Am. J. Physiol. (Gastrointest. Liver
Physiology).
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on 6 November 95