Relationship of simultaneous atrial and ventricular pressures in the stage 16 to 27 chick embryo. Hu, Norman, and Bradley B. Keller. NIH SCOR in Pediatric Cardiovascular Diseases, Strong Children's Research Center, Department of Pediatrics, Box 631, University of Rochester School of Medicine & Dentistry, 601 Elmwood Avenue, Rochester, New York
APStracts 2:0229H, 1995.
Ventricular filling is determined by a dynamic balance between atrial and ventricular load and function. The embryonic cardiovascular system undergoes simultaneous growth and morphogenesis at the cellular, tissue, and organ levels to match the embryo's geometrically increasing metabolic demands. As part of our long-term investigation of atrial/ventricular coupling during primary cardiac morphogenesis, we defined the relationship between simultaneous atrial and ventricular pressures in the stage 16 to 27 white Leghorn chick embryo. We measured atrial and ventricular blood pressures with servonull micropressure systems, and sampled analog waveforms digitally at 500 Hz. Peak atrial pressure increased geometrically from 0.38+/-0.03 to 1.21+/-0.17 mmHg, while ventricular end-diastolic pressure increased linearly from 0.18+/-0.03 to 0.55+/-0.04 mmHg. The passive and active mean pressure gradients increased from 0.23+/-0.04 mmHg and 0.20+/-0.03 mmHg at stage 16 to 0.52+/-0.10 mmHg and 0.62+/ -0.11 mmHg at stage 27, respectively. The atrioventricular (AV) pressure gradients were similar for stages 16, 18, and 21, then increased to stage 27. This diastolic pressure gradient identifies the AV orifice and developing endocardial cushions as a site of flow resistance that may influence both ventricular filling and chamber morphogenesis.

Received 31 January 1995; accepted in final form 22 May 1995.
APS Manuscript Number H89-5.
Article publication pending Am. J. Physiol. (Heart Circ. Physiology).
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on  8 June 1995.