Activation of angiotensin-converting-enzyme expression in the
infarct zone following myocardial infarction.
Passier, Robert C. J. J., Jos F. M. Smits, Monique J. A. Verluyten,
Roland Studer, Helmut Drexler, and Mat J. A. P. Daemen.
University of Limburg, Cardiovascular Research Institute
Maastricht, Departments of Pharmacology and Pathology, Maastricht,
The Netherlands; and Department of Molecular Cardiology, University
of Freiburg, Freiburg, Germany
APStracts 2:0189H, 1995.
In the present study we quantified ACE mRNA and localized ACE mRNA and
protein in the infarcted rat heart. Wistar rats underwent ligation of
the left descending coronary artery, resulting in myocardial
infarction (MI), or a sham-operation. At different times (1-90 days)
after surgery (n=3 each) the heart was removed and divided into right
ventricle (RV), septum (SE) and left ventricle (LV). ACE mRNA was
quantified by competitive reverse transcriptase polymerase chain
reaction (RT-PCR). At 4 and 7 days after MI we found a 2.8-fold
increase of ACE mRNA (n=3; p
Received 6 February 1995; accepted in final form 24 April 1995.
APS Manuscript Number H110-5.
Article publication pending Am. J. Physiol. (Heart Circ. Physiology).
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on 9 May 1995.