Kininogen changes in human plasma following a test meal or insulin
administration.
Rothschild, Adolfo M., Guenther Boden, and Robert W. Colman.
The Sol Sherry Thrombosis Research Center, Divisions of Hematology
and Endocrinology/Metabolism, and the General Clinical Research
Center, Temple University School of Medicine, Philadelphia,
Pennsylvania 19140
APStracts 2:0443H, 1995.
The effect of food intake and of insulin on plasma bradykinin (BK)
reserves [total (TK), high molecular weight kininogen (HK) and low
molecular weight kininogen (LK)] was followed by determining (by
ELISA) the amount of BK released by trypsin from plasma collected
prior to and after a test meal or hyperinsulinemic clamping. LK was
kininogen remaining in plasma after in vitro kaolin treatment, which
removes all HK. HK was the difference between TK and LK. Thirty
minutes after a test meal, TK and HK decreased by 8.0 + 0.6 and 39.7
+ 6.6%, respectively, in 6 out of 7 subjects. Return to prealimentary
levels occurred after 90-120 min. Hyperinsulinemia, comparable to
that arising after the test meal, partly reproduced such treatment's
effect on TK and HK, but did not affect blood or plasma kininogen in
vitro. Observed postprandial hypotension and increased leg blood flow
could be caused, in part, by BK released from plasma HK after
cleavage at vascular, possibly endothelial, sites activated by
insulin.
Received 2 March 1995; accepted in final form 21 August 1995.
APS Manuscript Number H203-5.
Article publication pending Am. J. Physiol. (Heart Circ. Physiology).
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on 6 November 95