Constancy and variability of contractile efficiency as a function of calcium and crossbridge kinetics: simulation. Yamaguchi, Hiroki, Miyako Takaki, Hiromi Matsubara, Shingo Yasuhara, and Hiroyuki Suga. Department of Physiology II, Okayama University Medical School, Shikatacho, Okayama, 700 JAPAN
APStracts 2:0444H, 1995.
We simulated myocardial Ca2+ (Ca) and crossbridge (CB) kinetics to get insight into the experimentally observed constancy and variability of cardiac "contractile efficiency" in generating "total mechanical energy" under various inotropic and pathological conditions. The simulation consisted of a Ca transient, Ca association and dissociation rate constants of troponin C, and CB on and off rate constants. We evaluated sarcomere-isometric twitch contractions at a constant muscle length. We assumed that each CB cycle hydrolyzes one ATP and that the "force-length area" (FLA) quantifies the total mechanical energy generated by CB cycles in a twitch contraction. FLA is a linear version of the "pressure-volume area" (PVA) which quantifies the total mechanical energy of cardiac twitch contraction and correlates linearly with cardiac oxygen consumption (Suga: Ventricular energetics. Physiol Rev 70: 247-277, 1990). The simulation shows that the contractile efficiency varies with changes in the Ca transient and Ca and CB kinetics except when they simultaneously speed up or slow down proportionally. These results point to possible mechanisms underlying the constancy and variability of cardiac contractile efficiency.

Received 22 June 1994; accepted in final form 22 September 1995.
APS Manuscript Number H550-4.
Article publication pending Am. J. Physiol. (Heart Circ. Physiology).
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on 6 November 95