Mechanisms of venous return and ventricular filling in elasmobranch fishes. Lai, N. Chin, Jeffrey B. Graham, Valmik Bhargava, and Ralph Shabetai. Cardiology Section, Veterans Administration Medical Center, San Diego, CA, Center for Marine Biotechnology and Biomedicine and Marine Biology Research Division, Scripps Institution of Oceanography, University of California, San Diego, La Jolla, CA 92093-204, Department of Medicine, University of California, San Diego, San Diego, CA
APStracts 2:0484H, 1995.
The current concept of ventricular filling in the elasmobranch fishes (sharks and rays) is that a subambient pericardial pressure establishes a negative diastolic pressure gradient for the atrium and that ventricular end-diastolic volume is exclusively determined by atrial systole. In contrast, recent findings using echo-Doppler and digital imaging techniques have demonstrated two filling phases in the elasmobranch ventricle. In this study, simultaneous atrial and ventricular pressure measurements made on sharks with either an open or intact pericardium establish that atrial pressure is above ventricular diastolic pressure until the onset of ventricular systole. A positive biphasic atrio-ventricular pressure gradient thus assures ventricular filling during early diastole, as a result of ventricular relaxation, as well as during atrial systole. Although a reduction in pericardial pressure resulted in a decline in both the atrial and ventricular pressure, a positive atrio-ventricular pressure gradient is conserved. The finding that atrial diastolic pressure is not lower than ventricular diastolic pressure, when combined with previous results showing that pericardial pressure is generally at or above ambient and that ventricular filling is biphasic, constitute a strong body of evidence favoring the operation of a direct venous inflow as the mechanism by which the elasmobranch heart fills.

Received 18 July 1994; accepted in final form 9 October 1995.
APS Manuscript Number H632-4.
Article publication pending Am. J. Physiol. (Heart Circ. Physiology).
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on 6 November 95