Sequential canine skeletal muscle ischemia: prior ischemia/
reperfusion results in contralateral muscle salvage.
Liauw, Shinta K., Barry B. Rubin, Thomas F. Lindsay, Alexander D.
Romaschin, and Paul M. Walker.
Departments of Vascular Surgery and Clinical Biochemistry, R.
Fraser Elliot Laboratory, The Toronto Hospital, University of
Toronto
APStracts 2:0426H, 1995.
Sequential muscle ischemia/reperfusion in a paired canine gracilis
muscle model results in a significant muscle salvage. In this model,
a randomly chosen first gracilis muscle is subjected to 5 hours of
ischemia followed by 48 hours of reperfusion in vivo. The
contralateral (second) gracilis muscle is then made ischemic for the
same duration as the first muscle. During this ischemic interval, the
first muscle is removed for necrosis determination. After 48 hours of
in vivo reperfusion, the second muscle is removed for necrosis
determination. A mean muscle salvage of 60% was achieved in the
second group of muscles using this protocol and is greater than any
previous salvage achieved using free radical scavengers (Walker, PM.
et al. Salvage of skeletal muscle with free radical scavengers. J.
Vasc. Surg. 5(1): 68-75, 1987) or neutrophil and plasma removal
during reperfusion (Rubin, B., et al. Phospholipid peroxidation,
deacylation and remodelling in post-ischemic skeletal muscle. Am. J.
Physiol. 263(32): H1695-H1702, 1992). Analysis of tissue adenine
nucleotides from muscle biopsies taken before ischemia, at end
ischemia and at 0.25, 4 and 48 hours of reperfusion indicated that
significant sparing of ATP utilization occurred in the second muscle
group during ischemia, consistent with increased tissue survival.
Preliminary analysis of tissue heat shock proteins (hsp) shows that
the second group of muscles have a different pattern of hsp
expression than the first group prior to the onset of ischemia. The
results suggest that reduced ATP utilization during ischemia and
altered hsp expression in the second muscle may play a role in the
tissue salvage observed in this sequential muscle ischemia model.
Received 18 January 1995; accepted in final form 14 September
1995.
APS Manuscript Number H45-5.
Article publication pending Am. J. Physiol. (Heart Circ. Physiology).
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on 31 October 95