Timing of exogenous surfactant administration in a rabbit model of acute lung injury. Ito, Yushi, John Goffin, Ruud Veldhuizen, Mariamma Joseph, Dave Bjarneson, Lynda McCaig, Li-Juan Yao, John Marcou, and Jim Lewis. Lawson Research Institute, St. Joseph's Health Centre, Departments of Physiology, Medicine, and Pathology, University of Western Ontario, London, Ontario, Canada N6A 4V2
APStracts 2:0535A, 1995.
The purpose of this study was to evaluate early versus late administration of exogenous surfactant in an adult rabbit model of acute lung injury. Lung injury was induced by repetitive whole lung saline lavage and subsequent mechanical ventilation. Bovine lipid extract surfactant was instilled either 1 hr (EARLY) or 4 hr (LATE) after the last lavage. Animals were monitored for 7 hr after the last lavage. Although PaO2 values increased significantly immediately after treatment in both the EARLY and LATE group, this improvement was not sustained in the LATE group. There was also a higher incidence of pneumothoraces in the LATE group versus both the EARLY group and a non-treated control group. The ratio of poorly functioning small surfactant aggregates to superior functioning large aggregates was higher in the LATE group compared to the EARLY group. Morphologic analysis revealed that early surfactant treatment prevented the progression of lung injury over time. We conclude that administration of exogenous surfactant at an early time point in lung injury resulted in superior responses compared to later treatments.

Received 5 June 1995; accepted in final form 10 November 1995.
APS Manuscript Number A588-5.
Article publication pending Journal of Applied Physiology.
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on 8 December 95