The effect of diet and training on muscle glycogen storage and
utilization in sled dogs.
Reynolds, Arleigh J., Laurent Fuhrer, Harris L. Dunlap, Mark Finke,
and Francis A. Kallfelz.
Departments of Clinical Sciences, Pathology, The College of
Veterinary Medicine, Cornell University, Ithaca, NY, Zero Kennels,
Bakers Mills NY, and The Pet Center Alpo Petfoods Inc., Allentown
PA
APStracts 2:0275A, 1995.
Two groups of 8 Alaskan Huskies fed either a high-fat (HFD; 60% kcal
from fat, 15% from carbohydrate) or a high-carbohydrate diet (HCD;
60% kcal from carbohydrate, 15% kcal from fat) performed standard
aerobic(1 hr at 4 m/sec on a 0% slope) and anaerobic(3 min at 6.7
m/sec on a 10% slope) tests before and after training. Before and
immediately after each exercise test, venous blood samples were
collected and analyzed for lactate (L) and pyruvate (P), and muscle
biopsies were obtained under local anesthesia from m. semitendinosus
and analyzed for total muscle glycogen concentration (TMG). Training
was associated with a significant increase in pre-exercise TMG in
both diet groups; this effect was most marked in HCD. There was no
effect of diet or training on TMG utilization during the aerobic
tests. The rate of TMG utilization during the anaerobic tests was
between 20 and 40 times greater than that measured during the aerobic
tests. The pre-post exercise change in TMG was dependant on pre
-exercise TMG in HCD and HFD for both anaerobic tests
(HCD:p<0.01, r = 0.81, HFD: p
Received 11 February 1994; accepted in final form 20 June 1995.
APS Manuscript Number A156-4.
Article publication pending Journal of Applied Physiology.
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on 11 July 1995.