Effects of glutamine on the immune system - influence of muscular
exercise and hiv infection.
Rohde, Thomas, Henrik Ullum, Jan Palmo Rasmussen, Jens Halkjar
Kristensen, Eric Newsholme, Bente Klarlund Pedersen.
The Copenhagen Muscle Research Centre. Laboratory of Infectious
Diseases, Rigshospitalet, University Hospital, Copenhagen, Denmark,
Laboratory of Clinical Physiology of Exercise, Department of Medicine
TTA, Rigshospitalet, University Hospital, Copenhagen, Denmark,
Cellular Nutrition Research Group, Department of Biochemistry,
University of Oxford, Oxford OX1 3QU
APStracts 2:0100A, 1995.
Glutamine increased the proliferative response and the lymphokine
-activated killer (LAK) cell activity of blood mononuclear cells
(BMNC), isolated from normal healthy subjects (n=6), in a dose
-dependent manner, with optimum at 0.3-1.0 mM. The relative fraction
of CD3+, CD4+, CD8+, CD14+, CD16+ and CD19+ cells were not changed by
glutamine at a concentration of 0.6 mM, except in the
phytohaemagglutinin (PHA) stimulated proliferation experiment, where
the fraction of CD4+, and therefore CD3+ cells, increased. The
natural killer (NK) cell activity was not influenced by glutamine.
HIV seropositive subjects (n=8), who performed concentric bicycle
exercise for 1 h at 75 % of VO2max, had an overall lower PHA
stimulated proliferative response, compared to the HIV seronegative
control group (n=7). The proliferation during exercise was lower in
both the HIV seropositive- and the HIV seronegative group. Adding
glutamine in vitro did not normalize the lower proliferation in the
HIV seropositiv group or the attenuated proliferation seen during
exercise in both groups.
Received 6 June 1994; accepted in final form 7 March 1995.
APS Manuscript Number A556-4.
Article publication pending Journal of Applied Physiology.
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on 21 March 1995.