Metabolic and vascular effects of circulating endothelin-1 during moderately heavy prolonged exercise. Ahlborg, Gunvor, Eddie Weitzberg, Jan Lundberg. Department of Clinical Physiology, Huddinge Hospital, and Departments of Anestesiology and Intensive Care, Karolinska Hospital, and Pharmacology, Karolinska Institute, Stockholm, Sweden.
APStracts 2:0067A, 1995.
The aims were to investigate (a) whether the suppressive effect of endothelin-1 (ET-1) on splanchnic blood flow, arterial glucagon, insulin and glucose as well as splanchnic glucose production at rest is overcome or prevails during exercise (Study I) and (b) the influence of exercise on circulating arterial ET-1 levels (Studies I and II). In Study I, six healthy subjects performed 2 h of exercise at 50% of their peak oxygen uptake on two occasions, preceded by intravenous infusion of either physiological saline or ET-1 (4 pmol.kg-1.min-1). Blood specimens were taken from catheters in the brachial artery and a central hepatic vein. The ET-1 levels rose 15 -fold during the infusion at rest. Splanchnic blood flow fell after ET-1 (p<0.01) and remained unchanged during exercise. Splanchnic glucose production was approximately 25% lower compared to control exercise values during the whole exercise period (p<0.01). Neither heart rate, arterial glucagon, insulin, catecholamines, renin, glucose, lactate, nor glycerol levels differed from in the control exercise. Splanchnic glycerol uptake was slightly lower (p<0.05 -0.01), but lactate uptake remained unchanged compared to controls. The calculated gluconeogenesis from these substrates did not differ from the corresponding control values. ET-1 levels rose approximately two-fold in the control exercise during the initial 20 min (p<0.01).

Received 8 June 1994; accepted in final form 16 February 1995.
APS Manuscript Number A566-4.
Article publication pending Journal of Applied Physiology.
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on  1 March 1995.