Lipocortin 1 distribution in bronchoalveolar lavage from healthy
human lung: effect of prednisolone.
Smith, Susan F., Teresa D. Tetley, Avijit K. Datta, Terence Smith,
Abraham Guz, and Roderick J. Flower.
Department of Medicine, Charing Cross and Westminster Medical
School, Fulham Palace Road, London, W6 8RF and Department of
Biochemical Pharmacology, The William Harvey Research Institute, St
Bartholomew's Hospital Medical College, Charterhouse Square, London,
EC1M 6BQ, UK
APStracts 2:0089A, 1995.
Lipocortin 1 (Lc1; annexin 1) may mediate some anti-inflammatory
actions of the glucocorticoids, probably following binding to
specific cell surface binding sites. We have quantified Lc1 levels in
bronchoalveolar lavage (BAL) fluid and cells collected from 7 healthy
volunteers before and after seven days of treatment with an oral
glucocorticoid, prednisolone (30mg/day). Extracellular BAL Lc1 was
higher and cellular Lc1 was lower after prednisolone than before
(Extracellular expressed as median[range], ng/mg albumin - before,
9.8[4.8-35.0]; after, 23.6[1.9-41.4], p<0.05. Cellular expressed as
median[range], ng/106 cells - before 23.3[14.6-26.9]; after,
18.0[12.2-26.8]; p<0.05). The distribution of Lc1 within BAL cells
ex vivo (cell surface = 25%, cytosol = 50%, membrane = 25%) was
unaffected by prednisolone treatment. However, in adherent cells
which had been cultured for 4h, 70-80% of the Lc1 was on the cell
surface. In summary, prednisolone appears to promote cellular release
of Lc1. The difference in distribution of cellular Lc1 in BAL cells
ex vivo and in vitro may reflect adherence and/or activation.
Received 5 December 1994; accepted in final form 27 February
1995.
APS Manuscript Number A1240-4.
Article publication pending Journal of Applied Physiology.
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on 21 March 1995.