Superoxide dismutase derivative reduces the oxidative damage in
skeletal muscle of rats during exhaustive exercise.
Radak, Zsolt, Katsumi Asano, Masayasu Inoue, Takako Kizaki, Shuji Oh
-Ishi, Keiichiro Suzuki, Naoyuki Taniguchi, and Hideki Ohno.
Laboratory of Exercise Physiology, Institute of Health and Sport
Sciences, University of Tsukuba, Tsukuba 305; Department of
Biochemistry I, Osaka City University Medical School, Osaka 545,
Department of Hygiene, National Defense Medical College, Tokorazawa
359; Department of Biochemistry, Osaka University Medical School,
Suita, 565, Japan
APStracts 2:0092A, 1995.
Superoxide dismutase derivative (SM-SOD), which circulates and is
bound to albumin with a half-life of 6 hr, was injected
intraperitoneally into rats before exhaustive treadmill running in
order to study its antioxidant scavenging capacity in the plasma, and
soleus and tibialis muscles. The exercise induced a marked increase
in xanthine oxidase (XO) activity in plasma and increase in
thiobarbituric acid-reactive substances (TBARS) in the plasma as well
as in the soleus and tibialis muscles of non-administered rats
immediately after the exercise. The immunoreactive content and
activity of both SOD isoenzymes (Cu,Zn-SOD and Mn-SOD) of the non
-administered rats, increased in the soleus and tibialis muscles
immediately after the running. The SM-SOD treatment definitely
attenuated the degree of the increase in TBARS and XO in all samples
examined immediately after exercise. Glutathione peroxidase activity
significantly increased in the soleus muscle of non-administered rats
one day after the running, whereas catalase activity remained
unchanged throughout the experimental period. These results suggest
that a single bout of exhaustive exercise induces oxidative stress in
skeletal muscle of rats and that this oxidative stress can be
attenuated by exogenous SM-SOD.
Received 5 December 1994; accepted in final form 3 March 1995.
APS Manuscript Number A1235-4.
Article publication pending Journal of Applied Physiology.
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on 21 March 1995.