Indomethacin does not alter the natural killer cell response to 2.
5 hours of running (revised manuscript #a1185-4).
Nieman, D. C., J. C. Ahle, D. A. Henson, B. J. Warren, J. Suttles, J.
M. Davis, K. S. Buckley, S. Simandle, D. E. Butterworth, O. R.
Fagoaga, S. L. Nehlsen-Cannarella.
Departments of Health, Leisure, and Exercise Science; Biology;
Appalachian State University, Boone, North Carolina 28608. Department
of Biochemistry, James H. Quillen College of Medicine, East Tennessee
State University, Johnson City, Tennessee 37614. Department of
Exercise Science, School of Public Health, University of South
Carolina, Columbia, South Carolina 29208.Immunology Center, Loma
Linda University Medical Center, Loma Linda, California 92350.
APStracts 2:0170A, 1995.
The effect of 2.5 hours of treadmill running at 75.6+/-0.9% VO2max on
natural killer cell cytotoxic activity (NKCA) was investigated in 22
experienced marathon runners (VO2max 57.9+/-1.1 ml.kg-1.min-1, age
38.7+/-1.5 yrs). Blood samples were taken before (0715 h) and
immediately after exercise (1000 h), with three more samples taken
during 6 h of recovery (1130, 1300, 1600 h). Ten sedentary controls
(34.7+/-1.0 ml.kg-1.min-1 , 45.3+/-2.3 yrs) sat in the laboratory
during testing and had their blood sampled at the same time points.
The pattern of change in NKCA over time was significantly different
between groups [F(4,27)=6.53, P=0.001], with the runner's NKCA
dropping 51-61% below pre-exercise levels throughout 6 h of recovery.
Preincubation of blood mononuclear cells in vitro with indomethacin
had no effect on the difference in pattern of change in NKCA between
groups [F(4,17)=8.59, P=0.001], and did not attenuate the post
-exercise reduction in the runners. When NKCA was adjusted on a per-NK
cell basis, group differences and the post-exercise decline in NKCA
were eliminated [F(4,80)=0.65,P=0.63]. Serum cortisol and plasma
epinephrine in the runners were elevated relative to controls during
recovery from exercise, but no significant correlation with changes
in NK cells or NKCA was found. These data indicate that NKCA is
decreased significantly during recovery from 2.5 h running due to a
numerical redistribution of NK cells.
Received 21 November 1994; accepted in final form 18 April 1995.
APS Manuscript Number A1185-4.
Article publication pending Journal of Applied Physiology.
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on 2 May 1995.