Prevention of ischemia reperfusion lung injury by inhaled nitric
oxide in neonatal piglets.
Barbotin-Larrieu, Francois, Michel Mazmanian, Bruno Baudet,
H[acute]el[grave]ene D[acute]etruit, Alain Chapelier, Jean-Marie
Libert, Philippe Dartevelle, Philippe Herv[acute]e, and The Paris-Sud
University Lung Transplantation Group.
Laboratoire de Chirurgie Exp[acute]erimentale, H[circumflex]opital
Marie Lannelongue, Universit[acute]e Paris-Sud, Le Plessis Robinson,
France, Unit[acute]e de R[acute]eanimation P[acute]ediatrique et
N[acute]eonatale, H[circumflex]opital Saint Vincent de Paul,
Universit[acute]e Paris V, Paris
APStracts 2:0456A, 1995.
Lung ischemia-reperfusion results in a decrease in the release of
nitric oxide (NO) by the pulmonary endothelium. NO may have lung
-protective effects by decreasing neutrophil accumulation in the lung.
We tested whether NO inhalation would attenuate reperfusion-induced
endothelium dysfunction and increases in microvascular permeability
and pulmonary vascular resistance by preventing neutrophil lung
accumulation. After baseline determinations of pulmonary vascular
resistance (PVR), coefficient of filtration (Kfc), and circulating
neutrophil counts, isolated neonatal piglet lungs were subjected to a
one-hour period of ischemia followed by a one-hour period of blood
reperfusion and reventilation with or without additon of NO (10 ppm).
NO prevented reperfusion-induced increases in PVR and Kfc, as well as
the decrease in circulating neutrophils. After reperfusion, increases
in Kfc were correlated to decreases in circulating neutrophils. NO
prevented reperfusion-induced decrease in endothelium-dependent
relaxation in precontracted pulmonary artery rings. This demonstrates
that inhaled NO prevents microvascular injury, endothelium
dysfunction and pulmonary neutrophil accumulation in a neonatal
piglet model of lung ischemia-reperfusion.
Received 16 June 1995; accepted in final form 4 October 1995.
APS Manuscript Number A641-5.
Article publication pending Journal of Applied Physiology.
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on 6 November 95