Heart, liver and skeletal muscle myeloperoxidase activity during
exercise.
Belcastro, Angelo N., Gavin D. Arthur, Tracie A. Albisser, and Daniel
A. Raj.
Laboratory of Cell Physiology and Exercise, School of
Rehabilitation Sciences, Faculty of Medicine, and School of Human
Kinetics, University of British Columbia, Vancouver, B.C., Canada,
V6T 2B5
APStracts 2:0501A, 1995.
The purpose of this study was to determine if contractile activity
associated with running exercise was a pre-requisite for neutrophil
infiltration into rat tissues. Hydrogen peroxide-dependent
myeloperoxidase activity (MPO) for rat (N=8) liver, heart and
gastrocnemius muscles were assayed after 58 +/- 11 minutes of running
to voluntary exhaustion (25m/min; 0% grade). MPO activities (mean +/-
S.D.) measured with 0.6 mM hydrogen peroxide (H2O2) were 0.988 +/-
0.331 (skeletal muscle), 1.563 +/- 0.303 (heart) and 1.652 +/- 0.510
(liver) U/g for control samples compared to 1.690 +/- 0.321, 3.128
+/- 1.221 and 2.752 +/- 0.437 U/g, respectively, for the exercise
group (p /= 0.05). Exercise did not alter the Km values for cardiac
and liver samples, however for skeletal muscle the Km was 28% lower
than control (p
Received 10 July 1995; accepted in final form 7 November 1995.
APS Manuscript Number A743-5.
Article publication pending Journal of Applied Physiology.
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on 30 November 95