Adaptation to eccentric exercise: effect on cd64 and cd11b/cd18
expression.
Pizza, Francis X., Bruce H. Davis, Steven D. Henrickson, Joel B.
Mitchell, John F. Pace, Nancy Bigelow, Paul Dilauro, and Thomas
Naglieri.
Department of Physical Education, Texas Christian University, Fort
Worth, Texas 76129, Analytical Cytometry Laboratory, Harris Methodist
Hospital, Fort Worth, Texas 76104
APStracts 2:0380A, 1995.
The primary purpose of the study was to examine circulating
neutrophils and monocytes and their plasma membrane expression of
CD64, CD11b, and CD18 following 2 bouts of eccentric exercise.
Subjects (N=10) performed 25 forced-lengthened contractions of the
forearm flexors on two occasions separated by 3 weeks. Blood samples
were obtained before exercise and at 1.5, 6, 12, 24, 48, 72, and 96 h
of recovery. CD64, CD11b, and CD18 expression was determined via
direct immunofluorescence and used as an indicator of neutrophil and
monocyte activation. Creatine kinase (CK) activity (B1=1390, B2=108
U_l-1), myoglobin (B1=163, B2=41 ng_dl-1), muscle soreness and
tenderness were higher (p<0.01) following the first bout (B1)
compared to the second bout (B2). Neutrophils at 6, 12, and 96 h were
higher (p<0.05) for B1 vs. B2. CD11b expression on neutrophils
was 2.7 fold higher at 72 h for B1 vs. B2. CD64 expression on
neutrophils at 72 and 96 h was 1.4 and 1.9 fold higher, respectively
for B1 vs. B2. At 72 and 96 h, CD18 and CD64 expression on monocytes
was 1.3 fold higher for B1 vs. B2. The observed changes were not
significantly correlated with changes in CK activity or myoglobin. In
conclusion, the adaptation to eccentric arm exercise was associated
with a reduction in circulating neutrophils and a lower state of
neutrophil and monocyte activation.
Received 12 December 1994; accepted in final form 1 August 1995.
APS Manuscript Number A1259-4.
Article publication pending Journal of Applied Physiology.
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on 15 September 1995.