Saccade-related adtivity in monkey superior colliculus; I. characteristics of burst and buildup cells. Munoz, Douglas P., Robert H. Wurtz. Laboratory of Sensorimotor Research, National Eye Institute, Bethesda, MD 20892-4435, USA and MRC Group in Sensory-Motor Physiology, Department of Physiology, Queen's University, Department of Physiology, Kingston, Ontario, Canada K7L 3N6.
APStracts 2:0072N, 1995.
SUMMARY AND CONCLUSIONS
1. In the monkey superior colliculus (SC), the activity of most saccade- related neurons studied so far consists of a burst of activity in a population of cells at one place on the SC movement map. In contrast, recent experiments in the cat have described saccade-related activity as a slow increase in discharge before saccades followed by a hill of activity moving across the SC map (Munoz et al. 1991). In order to explore this striking difference in the distribution of activity across the SC, we recorded from all saccade-related neurons that we encountered in microelectrode penetrations through the monkey SC, and placed them in categories according to their activity during the generation of saccades. 2. When we considered the activity preceding the onset of the saccade, we could divide the cells into two categories. Cells with burst activity had a high frequency discharge just before saccade onset but little activity between the signal to make a saccade and saccade onset. About two thirds of the saccade-related cells had only a burst of activity. Cells with a build up of activity began to discharge at a low frequency after the signal to make a saccade and the discharge continued until generation of the saccade. About one third of the saccade- related cells studied had a build up of activity, and about three quarters of these cells also gave a burst of activity with the saccade in addition to the slow buildup of activity. 3. The build up of activity seemed to be more closely -3- J229-4RR related to preparation to make a saccade than to the generation of the saccade. The build up developed even in cases when no saccade occurred. 4. The falling phase of the discharge of these saccade- related cells stopped with the end of the saccade (a clipped discharge), shortly after (partially clipped), or long after (unclipped). 5. Some cells had closed movement fields in which saccades that were substantially smaller or larger than the optimal amplitude were not associated with increased activity. Other cells tended to have open-ended movement fields without any peripheral border; they were active for all saccades of optimal direction whose amplitudes were equal to or greater than a given amplitude. We found both types of movement fields at all movement field eccentricities studied within the SC. 6. The activity of cells with open-ended movement fields did not result from the smear of the visual target as it swept across the retina during a saccade because the discharge of the cell was still present when saccades were made in the dark to remembered rather than visual targets. The activity of these cells was also not due to the occurrence of corrective saccades since the activity was visible whether or not there was one. 7. In penetrations through the intermediate layers of the SC, we usually found cells with a burst of activity and those with closed movement fields to lie more dorsally than those with -4- J229-4RR a build up of activity and open-ended movement fields. 8. We also compared the activity of the saccade- related cells to the activity of fixation cells located in the rostral pole of the SC (Munoz and Wurtz 1993). We found a transition between saccade-related cells with open-ended movement fields and fixation cells. Cells within this transition zone were tonically active during fixation but also discharged during small contraversive saccades. These fixation cells were encountered deeper in the intermediate layers, at the same level as the cells with open- ended movement fields and buildup of activity. We propose that fixation cells form a rostral extension of the layer of cells with a buildup of activity. 9. We conclude that these characteristics of the saccade- related cells overlap sufficiently to allow us to place the cells into two groups. Burst cells have a high frequency burst occurring immediately prior to saccades and no build up of activity, the majority have clipped activity at the end of the saccade, and usually have closed movement fields. In contrast, buildup cells show activity beginning with the signal to make a saccade that continues until the generation of the saccade, the majority have partially clipped activity at the end of the saccade, and have open-ended movement fields. Since we encountered the cells with burst activity and closed movement fields more dorsally than we did cells with buildup activity and open-ended movement fields, we hypothesize further that the burst and buildup cells can be regarded as separate functional -5- J229-4RR sublayers with the burst layer on top and the buildup layer below. The buildup cells are similar to the saccade-related cells in the cat SC, but the burst cells may be an added feature of the primate SC.

Received 29 April 1994; accepted in final form 13 February 1995.
APS Manuscript Number J229-4.
Article publication pending J. Neurophysiol.
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on  3 April 1995.