The effect of stimulation on burst firing in cat primary auditory cortex.
Bowman, Denise M., Jos J. Eggermont and Geoff M. Smith.
Behavioural Neuroscience Research Group, Department of Psychology, The
University of Calgary, Calgary, Alberta, Canada.
APStracts 2:0196N, 1995.
SUMMARY AND CONCLUSIONS
1. Neural activity was recorded extracellularly with two independent
microelectrodes, aligned in parallel, and advanced perpendicular to
isofrequency sheets in cat primary auditory cortex. Multi-unit activity was
separated into single unit spike trains using a maximum variance spike sorting
algorithm. Only units which demonstrated a high quality of sorting and a
minimum spontaneous firing rate of 0.2 spikes/sec were considered for
analysis. The primary aim of this study was to describe the effect of periodic
click train and broad band noise stimulation on short time scale (= 50 ms)
bursts in the spike trains of single auditory cortical units and to determine
whether stimulation influenced the occurrence, spike count, and/or temporal
structure of burst firing relative to a spontaneous baseline. 2.
Extracellular recordings were made in 20 juvenile and adult cats from 69
single auditory cortical units during click train stimulation and silence, and
from 30 single units during noise stimulation and in silence. In an additional
15 single units the effect of both click train and noise stimulation was
investigated. The incidence, spike count, and temporal structure of short time
scale burst firing in the first 100 ms following stimulus presentation was
compared to burst firing in the period starting 500 ms after stimulus
presentation and to spontaneous burst firing. In addition, the serial
dependence of interspike intervals within a burst was tested during periods of
stimulation. 3. Burst firing was present in the stimulation, post
stimulation, and spontaneous conditions. Longer bursts (consisting of 3 or
more spikes) were more commonly observed in the post-stimulation and
spontaneous conditions, than in stimulation conditions. This effect was most
pronounced during click stimulation. A period of elevated firing activity was
present in a subset of units 0.5 to 1.5 s following stimulus presentation,
indicating prolonged effects of stimulation on single unit firing behavior.
4. For both stimuli, the proportion of single unit responses composed of
bursts was significantly greater in post-stimulation and spontaneous periods
than during stimulation. Burst rate was higher in post-click train stimulation
and spontaneous periods than during periods of click stimulation. The
isolated-spike rate was significantly higher during periods of noise and click
stimulation than in the post-stimulation and spontaneous periods. 5. An
examination of the auto-correlograms and higher order interspike interval
histograms of single unit responses during click train stimulation indicated
that 25% of single unit spike trains contained an excess of brief first-order
intervals, and 14% of spike trains contained a shortage of long higher-order
interspike intervals relative to a spontaneous baseline. During noise
stimulation, 10% of single unit responses contained an excess of short
intervals relative to baseline. Interspike intervals of short duration bursts
were not serially dependent during periods of stimulation. 6 . A comparison
of the auto-correlograms and higher-order interval histograms of single unit
responses in the post-stimulation and spontaneous conditions indicated that
20% of single unit spike trains contained an excess of short first, second,
and third order intervals following stimulation. This subgroup of single units
could not be distinguished on the basis of the age of the animal or on the
depth at which the recording was made. 7. The low incidence of burst firing
during stimulation opposes the view that bursts serve as a mechanism to
emphasize or amplify particular stimulus related responses in the presence of
ongoing spontaneous activity in the primary auditory cortex. Moreover, there
is little evidence to support the notion that brief bursts represent neural
codes, as intraburst intervals are not serially dependent. It is suggested
that pyramidal burst firing may be an effective way to evoke post-synaptic
firing in inhibitory interneurons and subsequently reduce or inhibit firing
activity in neocortex in a self-stabilizing process.
Received 4 January 1995; accepted in final form 19 June 1995.
APS Manuscript Number J5-5.
Article publication pending J. Neurophysiol.
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on 30 July 1995.