CONTRACTILE PROPERTIES OF THE TONGUE MUSCLES: EFFECTS OF HYPOGLOSSAL NERVE
AND EXTRACELLULAR MOTONEURON STIMULATION IN RAT.
Gilliam, Edwin E., and Stephen J. Goldberg.
Department of Anatomy, POB 980709, Medical College of Virginia, Virginia
Commonwealth University, Richmond, Virginia 23298-0709.
APStracts 2:0108N, 1995.
SUMMARY AND CONCLUSIONS
1. Stimulation of the whole hypoglossal (XII) nerve or its medial or lateral
branch in the rat produced two major movements of the tongue as measured with
a single force transducer attached to the tip of the tongue. Stimulation of
the whole XII th nerve or the lateral branch produced a retrusion of the
tongue while stimulation of the medial branch produced a protrusion. 2. The
average retrusive twitch tension evoked by stimulation of the XII th nerve
(11.25 g) or lateral branch (12.02 g) was significantly greater (p=0.0001)
than the protrusive twitch tension (1.05 g) elicited by medial branch
stimulation. The tetanic tension produced by lateral branch stimulation (36.82
g) was significantly greater (p=0.007) than the whole nerve tetanic tension
(28.23 g). The greater tension elicited by stimulation of the lateral branch
of the nerve when compared to the tension elicited by stimulation of the whole
hypoglossal nerve was probably due to the absence of protrusive axons in the
lateral branch of the nerve. Stimulation of the whole hypoglossal nerve
activates axons innervating both protrusive and retrusive muscles, resulting
in a weaker net retrusive force. 3. The contraction time of the tongue in
response to medial branch stimulation (10.94 ms) was significantly faster than
when the whole XII th nerve (15.68 ms, p=0.007) or lateral branch (13.36 ms,
p=0.05) was stimulated. The twitch contraction time of the tongue in
response to whole hypoglossal nerve or lateral branch stimulation was not
significantly different. 4. The tongue appears to be generally fatigue
resistant in response to stimulation of the whole XII th nerve (Fatigue Index)
(FI-0.67), lateral nerve branch (FI-0.67) and medial nerve branch (FI-0.76).
5. Extracellular stimulation of motoneurons within the hypoglossal nucleus
elicited protrusive or retrusive movements with the medial branch of the nerve
intact. Protrusive movements were evoked by stimulation at sites generally
ventral in the nucleus, but rostral to the obex only, and were thought to be
caused by genioglossus motoneurons. The average protrusive twitch tension
elicited was 47.1 mg with a 16.0 ms twitch contraction time and a 1.1 FI.
Retrusive movements were evoked by stimulation at sites throughout the length
of the hypoglossal nucleus, generally ventral, and were thought to be caused
by intrinsic muscle motoneurons. The average retrusive twitch tension was 67.1
mg with a 18.5 ms twitch contraction time and a 0.97 FI. The difference in the
evoked protrusive and retrusive twitch tension was significant (p=0.0001) as
was the difference in twitch contraction time (p=0.01). 6. Extracellular
stimulation of motoneurons within the hypoglossal nucleus elicited only
retrusion with the lateral branch of the nerve intact. This retrusion was
elicited from nuclear stimulation sites generally dorsal in the nucleus, but
primarily rostral to the obex, and was presumed to be caused by
styloglossus/hyoglossus motoneurons. The average retrusive twitch tension was
86.0 mg with a 13.5 ms twitch contraction time and a 1.4 FI. This
styloglossus/hyoglossus motoneuron evoked twitch tension was significantly
stronger than that elicited by stimulation of presumed genioglossus
(p=0.0001) or intrinsic (p=0.0001) motoneurons. The
styloglossus/hyoglossus elicited twitch contraction time was significantly
faster than that elicited by stimulation of genioglossus (p=0.01) or
intrinsic (p=0.001) motoneurons. 7. The fatigue indices in response to
extracellular motoneuron stimulation were generally higher than those seen in
response to whole nerve or nerve branch stimulation.
Received 25 March 1994; accepted in final form 30 March 1995.
APS Manuscript Number J154-4.
Article publication pending J. Neurophysiol.
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on 1 May 1995.