Odor-induced currents in Xenopus olfactory receptor cells measured with
perforated-patch recording.
Zhainazarov, Aslbek B. and Barry W. Ache.
Whitney Laboratory and Depts. of Zoology and Neuroscience, University of
Florida, St. Augustine, FL 32086.
APStracts 2:0128N, 1995.
SUMMARY AND CONCLUSIONS
1.Odor-evoked currents were recorded in Xenopus laevis olfactory receptor
neurons (ORNs) using conventional, as well as nystatin and gramicidin-
perforated, whole-cell recording. The odor-evoked current ran down quickly in
conventional, but not in perforated, whole-cell recording. All three types of
recording gave similar values for the amplitude, latency, time to peak,
recovery time and reversal potential of the odor-evoked current. 2. A
secondary Cl current comprised a significant part of the odor-evoked current
(55-65%). E Cl measured by gramicidin perforation, which does not alter [Cl -
] i , was -2.3 +/- 5.0 mV, indicating that these neurons maintain a high [Cl -
] i and that the secondary Cl current plays an excitatory role in olfactory
transduction.
Received 2 March 1994; accepted in final form 11 April 1995.
APS Manuscript Number J137-5.
Article publication pending J. Neurophysiol.
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on 2 May 1995.