Alterations in pulmonary artery tone during repeated episodes of hypoxia. Mehta, B. C. Yang J. L. Departments of Medicine, University of Florida College of Medicine and the VA Medical Center, Gainesville, Florida
APStracts 2:0062L, 1995.
To examine the basis of pulmonary constriction during chronic hypoxia, rat pulmonary artery rings were precontracted and exposed to multiple episodes of hypoxia. First hypoxic episode resulted in a transient contraction, followed by potent relaxation, and then a slow sustained contraction. Repeated hypoxic exposure resulted in stronger initial contraction and attenuated relaxation. Prolongation of normoxic interval between hypoxic episodes reversed the attenuation of hypoxic relaxation. Pulmonary artery rings that were de-endothelialized or treated with the nitric oxide synthesis inhibitor Nw-nitro-L-arginine methyl ester or oxyhemoglobin displayed only hypoxic relaxation without initial or late contractions, and no attenuation of relaxation during repeated hypoxia. Pretreatment of rings with indomethacin, or adenosine or endothelin receptor antagonists had no effect on the hypoxia-mediated alterations. Thus repetitive exposure to hypoxia results in an increase in initial contraction and a decrease in relaxation of pulmonary artery rings. Frequency of hypoxic episodes and endothelial integrity determine pulmonary tone during repeated hypoxia. However, cyclooxygenase products, adenosine or endothelin do not play a role in hypoxia-mediated changes in rat pulmonary artery tone.

Received 9 January 1995; accepted in final form 17 April 1995.
APS Manuscript Number L004-5.
Article publication pending Am. J. Physiol. (Lung Cell. Mol.
Physiology).
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on 25 April 1995.