Interleukin-8 and monocyte chemoattractant protein-1 mrnas in
oxygen injured rabbit lung.
D'angio, Carl T., Robert A. Sinkin, Michael B. Lomonaco, and Jacob N.
Finkelstein.
Department of Pediatrics (Neonatology), Strong Children's Research
Center, University of Rochester, Rochester, NY 14642
APStracts 2:0013L, 1995.
The chemokines interleukin-8 (IL-8) and monocyte chemoattractant
protein-1 (MCP-1) are neutrophil and monocyte attractants,
respectively. We hypothesized that IL-8 and MCP-1 mRNA expression in
alveolar macrophages (AMs) lavaged from rabbit lung would be
increased by oxygen exposure, which is known to induce inflammation.
Adult rabbits were exposed to > 95% oxygen for up to 64 h and allowed
to recover in room air for up to 72 h before sacrifice by intravenous
pentobarbital injection and pulmonary lavage. Numbers of lavageable
polymorphonuclear cells (PMNs) and AMs rose during the exposure
protocol. Quantitative in situ hybridization with [3H]-labelled
cRNA probes showed both IL-8 and MCP-1 mRNA expression in AMs during
oxygen exposure, with peak levels of IL-8 mRNA at 56 h oxygen
exposure and of MCP-1 mRNA at 64 h oxygen exposure with 24 h room air
recovery. IL-8 mRNA was present in PMNs between 48 h oxygen exposure
and 64 h oxygen exposure with 24 h room air recovery. MCP-1 mRNA was
not expressed in PMNs. This pattern of chemokine mRNA expression
emphasizes the importance of inflammatory cells as effectors in the
pulmonary response to oxygen exposure.
Received 14 August 1994; accepted in final form 22 December 1994.
APS Manuscript Number L221-4.
Article publication pending Am. J. Physiol. (Lung Cell. Mol.
Physiology).
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on 24 February 1995.