Compartmentalization of glucose utilization after intravenous vs
intratracheal challenge with lipopolysaccharide.
Deboisblanc, Bennett P., Cornel Dobrescu, Nebojsa Skrepnik, Steve
Nelson, John J. Spitzer, Gregory J. Bagby.
Departments of Medicine and Physiology, Louisiana State University
Medical Center, New Orleans, Louisiana
APStracts 2:0177L, 1995.
The rate of glucose utilization (Rg) of various tissues including lung
and bronchoalveolar lavage (BAL) fluid cells was measured using the
2-deoxyglucose technique in Sprague-Dawley rats 4 hours after
challenge with either 1 mg/kg intravenous (i.v.) or 0.3 mg/kg
intratracheal (i.t.) lipopolysaccarrhide (LPS). After i.v. LPS, Rg
increased in whole lung and non-respiratory tissues, but was
unaltered in BAL cells. After i.t. LPS, the Rg of non-respiratory
tissues was unchanged but the Rg of BAL cells increased from 3.7+0.3
to 71.5+16.0 nmoles x min-1. This increase in the Rg of BAL cells was
explained by a doubling of the macrophage specific Rg, by a 100 fold
increase in polymorphonuclear leukocytes (PMN) number, and by a
higher Rg in PMN than in macrophages. These results demonstrate that
increased glucose utilization after i.t. LPS is confined to the
respiratory system and that intra-alveolar phagocytes participate in
this increase.
Received 28 March 1995; accepted in final form 1 October 1995.
APS Manuscript Number L96-5.
Article publication pending Am. J. Physiol. (Lung Cell. Mol.
Physiology).
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on 6 November 95