Clinical surfactant preparations mediate superoxide dismutase and
catalase uptake by type ii cells and lung tissue.
Nieves-Cruz, Bedford, Arnold Rivera, Javier Cifuentes, Gyorgy Pataki,
A. Keith Tanswell, Waldemar A. Carlo, Sadis Matalon, and Bruce
Freeman.
Departments of Pediatrics, Anesthesiology, Biochemistry and
Molecular Genetics, Physiology and Biophysics4, University of Alabama
at Birmingham, Birmingham, AL; Department of Paediatrics5, University
of Toronto, Ontario, Canada.
APStracts 2:0178L, 1995.
Pulmonary surfactant mixtures are rapidly taken up by alveolar type II
cells and thus may serve as vectors for the pulmonary delivery of
antioxidants enzymes to the alveolar epithelium. We prepared
emulsions of Survanta O with superoxide dismutase (CuZn, SOD) and
catalase (Cat) and quantified their cellular uptake both in vitro and
in vivo. Incubations of fetal lung epithelial cells with an emulsion
of Survanta O plus SOD and catalase mixtures resulted in significant
augmentation of SOD and catalase activities (12.8 +/- 4.6 SOD/[mu]g
DNA; 7.49 +/- 2.21 catalase/[mu]g DNA). These numbers were
significantly greater than those obtained in controls (1.8 U
SOD/[mu]g DNA; 0.55 +/- 0.52 U catalase/[mu]g DNA), Survanta O alone
(0.43 U SOD/g DNA; 0.16 U catalase/g DNA) and SOD and catalase alone
(3.47 +/- 5.2 U SOD/[mu]g DNA; 4.24 +/- 3.0 U catalase/[mu]g DNA).
Intratracheal instillation of the Survanta plus SOD and catalase
mixture resulted in significant augmentation of enzymes by the rat
lung homogenates. Confocal microscopic analysis revealed the presence
of antioxidant enzymes in the cytoplasm of epithelial cells. We
concluded that Survanta O supplementation, in addition to
replenishing surfactant stores, can also enhance the delivery of
antioxidant enzymes to alveolar epithelium both in vitro and in vivo.
Received 18 November 1994; accepted in final form 15 August 1995.
APS Manuscript Number L333-4.
Article publication pending Am. J. Physiol. (Lung Cell. Mol.
Physiology).
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on 6 November 95