Nitration of surfactant protein a (sp-a) results in decreased
ability to aggregate lipids.
Haddad, Imad Y., Sha Zhu, Harry Ischiropoulos, and Sadis Matalon.
Departments of Pediatrics, Anesthesiology, and Physiology and
Biophysics, University of Alabama at Birmingham, Birmingham, AL
35233-6810 and Institute for Environmental Medicine, University of
Pennsylvania, Philadelphia, PA 19104
APStracts 2:0182L, 1995.
We assessed the extent to which nitration of SP-A, isolated from the
bronchoalveolar lavage of patients with alveolar proteinosis, alters
its ability to enhance lipid aggregation, bind lipids and act
synergistically with surfactant apoproteins B and C (SP-B, SP-C) in
lowering the surface activity of surfactant lipids. SP-A was treated
with various concentrations of tetranitromethane (TNM) at pH 6, 7.4,
8, or 10. Depending on the pH, TNM acts either as nitrating (pH
>/=7.4), or an oxidizing agent (pH
Received 31 May 195; accepted in final form 20 September 1995.
APS Manuscript Number L177-5.
Article publication pending Am. J. Physiol. (Lung Cell. Mol.
Physiology).
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on 6 November 95