Effect of cold preservation on pulmonary arterial smooth muscle
cells.
Hall, Susan M, and Sheila G Haworth.
Developmental Vascular Biology & Pharmacology Unit, Institute of
Child Health, 30 Guilford Street, London, WC1N 1EH, Professor Sheila
G Haworth, Institute of Child Health, 30 Guilford Street, London WC1N
1EH
APStracts 2:0186L, 1995.
The efficacy of preservation fluids on the cytoskeleton and
contractile function of porcine pulmonary arterial smooth muscle (SM)
cells during cooling and rewarming was evaluated using EuroCollins
(EC), University of Wisconsin (UW), Marshalls solution (MS) and
tissue culture growth medium (GM). Functional studies included
passive distensibility and contraction to PGF2 in arterial rings and
wrinkling of silicone membranes by cooled/rewarmed cultured SM cells.
Immunofluorescence measurements were made of actin brightness in
cooled arterial rings. Cultured SM monolayers were stained with
antibodies to -SM actin, smooth muscle myosin and tubulin. Cooling:
All solutions resulted in increased arterial distensibility while EC
and MS reduced cell wrinkling. Using all solutions actin cables
thinned, myosin filaments dissociated and microtubules depolymerised.
Rewarming: Resistance to imposed tension increased in all arterial
rings. After GM, EC and MS preservation contraction to PGF2[alpha]
increased. Wrinkling increased and actin/myosin cables shortened
after GM and EC; after UW wrinkling decreased and actin/myosin cables
thinned. No recovery occurred after MS. Thus the type of preservation
solution influenced contractility during preservation and after
rewarming. The absence of spontaneous contraction in cells cooled in
UW may be advantageous.
Received 22 August 1994; accepted in final form 26 September
1995.
APS Manuscript Number L245-4.
Article publication pending Am. J. Physiol. (Lung Cell. Mol.
Physiology).
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on 6 November 95