Spontaneous release of submucosal gland serous and mucous cell
macromolecules from human nasal explants in vitro.
Ali, Mushtaq, Jennifer Maniscalco, James N. Baraniuk.
Division of Rheumatology, Immunology and Allergy, GL-020 Gorman
Building, Georgetown University, 3800 Reservoir Road, Washington,
D.C. 20007-2197, TEL (202) 687-8233, FAX (202) 687-8579
APStracts 2:0187L, 1995.
Respiratory epithelial and gland cells cultured in vitro demonstrate
changes from differentiated "serous" and "mucous"
cells towards intermediate "seromucous" cells. This
spontaneous process was examined by culturing human nasal mucosal
explants in CMRL-1066 media without growth factors for six days, and
measuring the concentrations of spontaneously released serous cell
products (lactoferrin, lysozyme, 7F10-immunoreactive
mucoglycoconjugates [7F10-irm]) and Alcian Blue staining mucous cell
products. 7F10-irm was progressively and significantly increased on
each day of culture. In contrast, lysozyme, lactoferrin, and Alcian
Blue staining material decreased significantly. Each had its own
pattern of decreasing release. Dexamethasone (1 [mu]M) had no effect
on these trends. PMA (100 nM) reduced 7F10-irm release on Days 4 to
6, and delayed the drop in lactoferrin release. Dexamethasone blunted
these effects of PMA. These data indicate that respiratory secretory
cells alter their phenotypes when cultured in vitro, and
progressively change the relative amounts of mucoglycoconjugates and
proteins spontaneously released. These changes should be anticipated
when interpreting experiments involving cultured respiratory cells.
Received 19 April 1995; accepted in final form 6 October 1995.
APS Manuscript Number L120-5.
Article publication pending Am. J. Physiol. (Lung Cell. Mol.
Physiology).
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on 6 November 95