Expression of the tyrosine phosphatase lar-ptp2 in rat lung is
confined to proliferating epithelia lining the airways and air
sacs.
Kim, Hyeja, Herman Yeger, Robin Han, Megan Wallace, Barry Goldstein,
and Daniela Rotin.
MRC Group in Lung Development, the Hospital For Sick Children, and
the Departments of Pediatrics and Clinical Biochemistry, University
of Toronto, 555 University Avenue, Toronto, Ontario, M5G 1X8. (2)
From the Dorrance H. Hamilton Research Laboratories, Division of
Endocrinology and Metabolic Diseases, Department of Medicine,
Jefferson Medical College, Thomas Jefferson University, 1020 Locust
St Philadelphia, PA, 19107
APStracts 2:0195L, 1995.
The LAR family tyrosine phosphatase LAR-PTP2B (RPTPs) was previously
shown to be expressed in the central and peripheral nervous system.
Here we show that LAR-PTP2, the larger alternatively spliced form of
the gene, is expressed in proliferating undifferentiated lung
epithelia in a developmentally regulated manner. Using in situ
hybridization and parallel immunostaining with PCNA to detect
proliferating cells, we demonstrate that LAR-PTP2 is expressed
exclusively in the undifferentiated epithelial cell layer lining the
bronchi, bronchioles and air sacs in late fetal development and in
the neonatal lung. These cells correspond to Clara and fetal alveolar
type II cells, as determined by parallel immunostaining with an
antibodies to surfactant proteins A and B. LAR-PTP2 expression
declined progressively with postnatal development, and by adult stage
there was no detectible expression in the airways or in the distal
(type I and II) mature non proliferating alveolar epithelial cells.
These results suggest that LAR-PTP2 may be involved in the regulation
of epithelial cell proliferation/differentiation during lung
development.
Received 15 September 1995; accepted in final form 18 October
1995.
APS Manuscript Number L275-5.
Article publication pending Am. J. Physiol. (Lung Cell. Mol.
Physiology).
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on 6 November 95