Insights Into Cellular Energy Metabolism From Transgenic Mice.
Koretsky, Alan P.
Dept. of Biological Sciences and Pittsburgh NMR Center for Biomedical
Research, Carnegie Mellon University, Pittsburg, Pennsylvania.
APStracts 2:0007P, 1995.
ABSTRACT
Rapid progress in manipulating the mouse genome now makes it possible to
express any cloned gene in virtually any cell type of the mouse. Homologous
recombination in embryonic stem cells allows the specific mutation of any
gene. These tools are finding widespread application to problems in cell
biology and physiology. After a brief description of some of the molecular
genetic techniques available for the mouse, the application of transgenic mice
to problems in cellular energy metabolism is discussed. The effects of
increasing glucose transport on carbohydrate metabolism in muscle and fat are
presented. Experiments using a transgenic mouse expressing creatine kinase in
liver to understand ATP catabolism and regulation of oxidative phosphorylation
are discussed. Finally, mice either lacking or misexpressing different
isoforms of creatine kinase have been useful for understanding the detailed
role of this important enzyme in cellular energy metabolism.
APS Manuscript Number P-0006-5.
Article publication scheduled October 1995 Physiological Reviews.
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on 23 September 1995.