APStracts 2:0033R, 1995.

Natriuretic peptide receptors are expressed during cerebral growth in the fetal rat. Brown, J., and Z. Zuo. Dr J. Brown, Physiological Laboratory, University of Cambridge, Downing Street, Cambridge CB2 3EG. Telephone No.: (0223) 333851, Fax No.: (0223) 333840

APStracts 2:0033R, 1995.

Autoradiography of frozen sections of fetal rat brain shows that receptor-like binding sites for atrial and C-type natriuretic peptides (ANP and CNP) occur in the generative juxtaventricular zone of the telencephalon after the twelfth embryonic day (E12). These sites avidly bind both ANP and CNP. They thus resemble the cloned NPR-C natriuretic peptide receptor. Covalent cross-linking of 125I -tyro-CNP(1-22) and 125I-ANP(1-28) to membrane proteins from E16 telencephala labels a single protein band on reducing sodium dodecyl sulphate (SDS)-polyacrylamide gel electrophoresis. The protein has high affinities for ANP and CNP, and a molecular mass of 60-70 kDa under reducing conditions, consistent with reduced NPR-C. However, the telencephalic protein has unusual physico-chemical properties in SDS under non-reducing conditions so it was not possible to assess whether this protein can form disulphide-bridged dimers like NPR-C.

Received 27 June 1994; accepted in final form 27 January 1995.
APS Manuscript Number R341-4.
Article publication pending Am. J. Physiol. (Regulatory Integrative
Comp. Physiology).
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on 25 February 1995.