Sympathetic vasoconstrictive responses to high and low sodium diets in diabetic and normal subjects. Miller, Judith A., With The Expert Technical Assistance Of Julie C. Dionne. Department of Medicine, University of Toronto, Canada
APStracts 2:0042R, 1995.
Uncomplicated insulin dependent diabetes mellitus (IDDM) has been associated with both a suppression of the reflex response to stimuli which activate the sympathetic nervous system (SNS) and an enhanced pressor response to catecholamines. The aim of these experiments was to examine cardiovascular regulation by the SNS in subjects with uncomplicated IDDM within 5 years of diagnosis to determine the responsible mechanism within the cardiopulmonary baroreflex arc, and the potential role played by extracellular fluid volume (ECFV) expansion. The integrity of the reflex arc was tested by examining the plasma norepinephrine (PNE) and forearm vascular (FVR) responses to cardiopulmonary baroreceptor deactivation by non-hypotensive Lower Body Negative Pressure (LBNP). The integrity of the effector limb was tested by measuring the PNE, FVR and mean arterial pressure (MAP) response to the Cold Pressor Test (CPT), a non-baroreceptor-mediated stimulus. The postganglionic neuroeffector junction was tested by measuring the venoconstrictive response to local norepinephrine infusion. ECFV was varied by altering the content of sodium in the diet. In subjects with IDDM, PNE and FVR responses to cardiopulmonary baroreceptor deactivation were attenuated, the MAP, FVR, and PNE responses to the CPT were intact, and the peripheral vasculature was hyper-responsive to norepinephrine infusion compared to control subjects. Sodium restriction resulting in ECFV contraction and reductions in central venous pressure normalized the FVR response to LBNP and the venous response to norepinephrine infusion in subjects with IDDM, without affecting the response in control subjects. In subjects with IDDM, the PNE response both to LBNP and the CPT remained attenuated with sodium restriction, even though other measures of sympathetic function were normalized. These data indicate that 1) sympathoinhibition in subjects with early IDDM may be a normal reflex response to increased cardiac filling pressures, mediated by cardiopulmonary baroreceptors; 2) sodium restriction normalizes both the cardiopulmonary baroreflex control of FVR and the hyper-reactivity of the peripheral vasculature to norepinephrine infusion; and 3) the measurement of PNE alone is inadequate to assess SNS activity in diabetic patients.

Received 10 September 1994; accepted in final form 27 January
1995.
APS Manuscript Number R505-4.
Article publication pending Am. J. Physiol. (Regulatory Integrative
Comp. Physiology).
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on 25 February 1995.