Increased lipolysis to beta adrenergic stimulation following
dehydroepiandrosterone treatment in rats.
Tagliaferro, A R, A M Ronan, J Payne, L D Meeker, S Tse.
Department of Animal and Nutritional Sciences, Human Nutrition Laboratory,
and Department of Mathematics, University of New Hampshire, Durham, NH
03824
APStracts 2:0004R, 1995.
Young adult male rats were treated with 4 mg DHEA per 100 gm diet for four
weeks, or were fed the same purified diet unadulterated (51 CHO:20 fat: 23.5
protein w/w). After 1 week, body weight and fat mass of the DHEA-fed rats
were significantly smaller than the controls. By the end of week 3, fat-free
mass of the DHEA rats was less than the controls. Neither food intake nor
resting metabolism, measured by indirect calorimetry, was different between
groups. Isolated epididymal adipocytes of DHEA rats were significantly
smaller and isoproterenol (x 10-7 M) stimulation of glycerol release was 53%
greater (P < 0.01) than the controls. Basal rate of glycerol release
increased significantly for both groups in response to the adenosine
inhibitor, adenosine deaminase; there were no significant interaction
effects. Inhibition of lipolysis by the adenosine analog,
phenylisopropyladenosine, was similar between groups. Findings support the
hypothesis that DHEA reduces adiposity directly by increased lipolysis, but
the mechanism of action does not involve a change in the antilipolytic
function of adenosine.
Received 27 December 1993; accepted in final form 6 January 1995.
APS Manuscript Number R702-3.
Article publication pending Am. J. Physiol. (Regulatory Integrative Comp.
Physiology).
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on 25 February 1995.