Vasopressin responses to corticotropin-releasing factor and
hypertonicity after truncal vagotomy in dogs.
Raff, Hershel, Paula E. Papanek, and Verne E. Cowles.
Departments of Physiology, Medicine and Surgery, Medical College of
Wisconsin; Surgical Research Program, Zablocki Veterans
Administration Medical Center; Physical Therapy Program, Marquette
University; and Endocrine Research Laboratory, St. Luke's Medical
Center, Milwaukee, WI 53215
APStracts 2:0209R, 1995.
Infusion of corticotropin-releasing factor (CRF) augments the plasma
vasopressin response to infusion of hypertonic saline in conscious
dogs. Furthermore, afferent vagal nerve input from the abdomen is
involved in the control of vasopressin release and may be altered by
CRF. The purpose of the present study was to characterize the effect
of CRF on the vasopressin response to hypertonic saline and to
determine if it is mediated by afferent input carried from the
abdominal vagus. Conscious, male dogs (n=5) underwent infusion of
isotonic saline (vehicle), CRF (10 or 20 ng/kg/min), hypertonic
saline (0.2 mmol/kg/min), or the combination of CRF and hypertonic
saline. Hypertonic saline increased plasma sodium from 147+/-1 to
153+/-1 meq/L and plasma vasopressin from 2.5+/-0.1 to 5.8+/-0.4
pg/ml. CRF infusion alone had no effect on plasma vasopressin. The
addition of 10 or 20 ng/kg/min CRF augmented the vasopressin response
to hypertonic saline to 7.7+/-1.7 and 6.9+/-0.3 pg/ml, respectively.
Truncal vagotomy did not attenuate the vasopressin response to
hypertonic saline without or with CRF infusion. We conclude that CRF
augments the vasopressin response to hypertonic saline and that this
effect is not mediated via afferents from the abdominal vagus.
Received 3 April 1995; accepted in final form 18 July 1995.
APS Manuscript Number R223-5.
Article publication pending Am. J. Physiol. (Regulatory Integrative
Comp. Physiology).
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on 30 July 1995.