Thermal and behavioral effects of lipopolysaccharide and influenza
in interleukin-1[beta] deficient mice.
Kozak, Wieslaw, Hui Zheng, Carole A. Conn, Dariusz Soszynski, Lex H.
T. Van Der Ploeg, Matthew J. Kluger.
Institute for Basic and Applied Medical Research, The Lovelace
Institutes, 2425 Ridgecrest Dr. S.E., Albuquerque, New Mexico 87108,
Department of Genetics and Molecular Biology, Merck Research
Laboratories, Division of Merck & Co., Inc., P.O. Box 2000,
Rahway, New Jersey 07065
APStracts 2:0151R, 1995.
Kozak, Wieslaw, Hui Zheng, Carole A. Conn, Dariusz Soszynski, Lex H.T.
Van der Ploeg, Matthew J. Kluger. Thermal and behavioral effects of
lipopolysaccharide and influenza in interleukin-1[beta] deficient
mice. Am. J. Physiol. (Regulatory Integrative Comp. Physiol.). This
study characterized body temperature (Tb), locomotor activity (Act),
and feeding behavior under normal conditions and following injection
with lipopolysaccharide (LPS) or inoculation with live influenza
virus of transgenic C57/black mice deficient in interleukin-1[beta]
(IL-1[beta]). Tb and Act in freely moving mice were measured by
biotelemetry. Mice deficient in IL-1[beta] had normal circadian
rhythm of Tb; however, they were less active than their control
counterparts. Mice injected ip with LPS (2.5 mg/kg) responded with a
prompt decrease of Tb, which lasted ca. 10 h, followed by a fever in
which the Tb reached a peak at 24 h post injection. There was no
difference between groups in the early drop of Tb after the LPS;
however, the 24 h peak of the Tb was lower in IL-1[beta] deficient
mice. The anorexic effects of LPS and influenza infection were
similar in both groups of mice. Mice given influenza virus (17.5 PFU,
LD50) gradually decreased Tb and Act. The fall of Tb was smaller in
the transgenic mice. The mice deficient in IL-1[beta] displayed
higher mortality rate due to influenza infection than the control
mice. We conclude that deficiency in IL-1[beta] results in lower
fever following the LPS injection and in impairment of the defense
response to infection with influenza.
Received 26 January 1995; accepted in final form 18 May 1995.
APS Manuscript Number R65-5.
Article publication pending Am. J. Physiol. (Regulatory Integrative
Comp. Physiology).
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on 8 June 1995.