Abdominal surgery-induced inhibition of gastric emptying is
mediated in part by interleukin-1[beta].
Coimbra, Claudia R., and Victor Plourde.
Neurobiology and Digestive Motility Laboratory, Andr[acute]e
-Viallet Clinical Research Center, University of Montr[acute]eal
APStracts 2:0279R, 1995.
The role of Interleukin-1B (IL-1[beta]) in abdominal surgery-induced
inhibition of gastric emptying was investigated. Abdominal surgery
was performed under halothane anesthesia and 5 min later, the 20 min
rate of gastric emptying was measured by the phenol red method in
conscious animals. In non-operated animals, i.v. IL-1[beta] dose
dependently decreased gastric emptying from 55.1 +/- 1.7 % in
controls down to 8.0 +/- 3.5 % (p<0.05) after treatment with IL
-1[beta] 1?[mu]g. Prior administration of IL-1[beta]ra (200 [mu]g)
completely abolished the inhibitory effects of IL-1[beta]. Surgery
inhibited gastric emptying by 83.4 % compared with rats receiving
anesthesia alone. IL-1[beta]ra (200 [mu]g) reversed the inhibition of
gastric emptying by 34.8 % (p<0.05) in the early (30 min) post
-operative period and by 32.3 % (p<0.05) in the late (120 min)
post-operative period. Use of Calcitonin-Gene-related-Peptide 8-37
(CGRP 8-37) in combination with IL-1[beta]ra in operated animals
resulted in no further reversal in the inhibition of gastric
emptying: CGRP 8-37-treated animals = 42.1 +/- 4.1 % ; CGRP 8-37 +
IL-1[beta]ra = 38.0 +/- 4.4 % (NS). Moreover, in non-operated
animals, CGRP 8-37 completely abolished the effects of i.v. IL
-1[beta] on gastric emptying: IL-1[beta]-treated animals = 11.1 +/-
2.0 % ; IL-1[beta] + CGRP 8-37 = 40.6 +/- 6.4 % (p<0.05). These
results suggest that IL-1[beta] is mediating part of the gastric
ileus observed after abdominal surgery, through the release of CGRP
from the visceral afferents.
Received 23 March 1995; accepted in final form 2 October 1995.
APS Manuscript Number R193-5.
Article publication pending Am. J. Physiol. (Regulatory Integrative
Comp. Physiology).
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on 14 November 95