Renal sensory receptor activation causes prostaglandin dependent release of substance p.. Kopp, Ulla C., Donna M. Farley, and Lori A. Smith. Departments of Internal Medicine and Obstetrics/Gynecology, University of Iowa College of Medicine and Department of Veterans Affairs Medical Center, Iowa City, Iowa 52242
APStracts 2:0299R, 1995.
Renal mechanoreceptor (MR) activation by increased ureteral pressure (UP) results in an increase in afferent renal nerve activity (ARNA) that is blocked by substance P receptor blockade and prostaglandin (PG) synthesis inhibition. To examine the interaction between substance P and PGs, the release of substance P and PGE into the renal pelvis was studied before and during renal pelvic perfusion with indomethacin. Before indomethacin, UP increased ARNA 43+/-6% and renal pelvic release of substance P from 11+/-3 to 29+/-8 pg/min and PGE from 319+/-71 to 880+/-146 pg/min. Indomethacin blocked the increases in ARNA and release of substance P and PGE produced by UP. Time control experiments showed reproducible increases in ARNA and release of substance P and PGE during UP. Mechanical stimulation of the renal pelvic wall in vitro resulted in an increase in PGE release, from 110+/-8 to 722+/-152 pg/min, that was abolished by indomethacin, suggesting a de novo PGE synthesis. The data suggest that UP results in a renal pelvic release of PGE which facilitates the release of substance P and activation of renal pelvic MR.

Received 27 July 1995; accepted in final form 23 October 1995.
APS Manuscript Number R469-5.
Article publication pending Am. J. Physiol. (Regulatory Integrative
Comp. Physiology).
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on 14 November 95