Effect of nacl load administered to the fetus on the bidirectional movement of 51cr-edta across the rat placenta. J., Tulc, and Tulcov[acute]a B. Department of Pharmacology, 2nd Faculty of Medicine and Department of Physiology, 1st Faculty of Medicine, Charles University, Prague, Czech Republic
APStracts 2:0304R, 1995.
A hypothesis that in the rat fluid circulates across the placenta, the circulation being maintained by active transport of Na+ from mother to fetus, has been tested. The hypothesis predicts that increasing Na+ supply to the fetus would increase the circulation of fluid which would increase convective flow of extracellular probes in the fetal -maternal direction. Clearance of 51Cr-EDTA from mother to fetus, Kmf, and from fetus to mother, Kfm, has been measured in anesthetized rats. Immediately before the transfer measurement the fetuses were injected intravenously with 10 [mu]l of isotonic glucose (controls) or with 30 [mu]l or 300 [mu]l of isotonic saline, or with 10 [mu]l, 30 [mu]l or 60 [mu]l of 9% solution of NaCl. In controls Kmf of 51Cr -EDTA was 2.0 +/- 0.7 [mu]l min-1, and Kfm was 4.3 +/- 1.0 [mu]l min -1. Injecting the fetus with NaCl had no effect on Kmf while it increased Kfm significantly in a dose dependent way. These observations support the hypothesis tested.

Received 10 March 1995; accepted in final form 1 November 1995.
APS Manuscript Number R196-5.
Article publication pending Am. J. Physiol. (Regulatory Integrative
Comp. Physiology).
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on 30 November 95