Photoperiod and population density interact to affect reproductive, adrenal, and immune function in male prairie voles (microtus ochrogaster). Nelson, Randy J., Joshua B. Fine, Gregory E. Demas, and Christopher A. Moffatt. Department of Psychology, Behavioral Neuroendocrinology Group, and Department of Population Dynamics, Reproductive Biology Division, The Johns Hopkins University, Baltimore, MD 21218-2686 USA
APStracts 2:0263R, 1995.
Seasonal breeding of rodents is often associated with changes in adrenal function; altered adrenal function could account, in part, for seasonal changes in immune function, and ultimately, influence seasonal fluctuations in survival. Animals commonly monitor the annual change in photoperiod to ascertain the time of year, and to make appropriate seasonal adjustments in physiology and behavior. Several extrinsic factors affect reproductive responsiveness to photoperiod. The interaction between population density and reproductive and adrenal responsiveness to photoperiod was assessed in the present experiment. Adult male prairie voles (Microtus ochrogaster) were maintained individually for 10 weeks in long (LD 16:8) or short (LD 8:16) photoperiods in rooms with either high (10.96 animals/m3) or low (0.18 animals/m3) population densities. Regardless of population density, short-day voles regressed the size of their reproductive organs; reproductive organ masses were higher in long-day voles housed in high density as compared to low density rooms. Paired adrenal masses were reduced in short-day voles, but were unaffected by population density; serum corticosterone concentrations were significantly elevated in short-day as compared to long-day animals. In both photoperiods, basal blood corticosterone levels were higher in voles from low density, as compared to high density rooms. Splenic masses were unaffected by day length, but were elevated among high density animals. Similarly, serum IgG levels were elevated among high density animals. These results suggest that population density per se, in the absence of behavioral interactions, can affect reproductive size, and possibly function, in long day conditions, and that prairie voles, which are highly social, exhibit higher corticosterone and lower IgG levels in low as compared to high densities. These results may be important in understanding arvicoline population fluctuations, as well as improving animal husbandry practices in the lab.

Received 1 February 1995; accepted in final form 12 September
1995.
APS Manuscript Number R85-5.
Article publication pending Am. J. Physiol. (Regulatory Integrative
Comp. Physiology).
ISSN 1080-4757 Copyright 1995 The American Physiological Society.
Published in APStracts on 31 October 95